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. 2017 Oct 17;16:162. doi: 10.1186/s12943-017-0729-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Further experiments confirmed the ceRNA role of HOTTIP. a & b & c Co-transfection dual luciferase reporter assay was used to demonstrate the ceRNA role of HOTTIP. d & e RNA pull-down and SDS-page assay showed HOTTIP may interact with Ago2 protein. f RIP experiments showed that Ago2 had a function in the formation of HOTTIP and miR574-5p complex and in their regulation of EZH1. *, P < 0.05; **, P < 0.01