Fig. 9.

Immunohistochemical analysis of seahorse brood pouch with anti-haCTL II and anti-haCTL IV antibodies. Sections of late-stage brood pouch were immunostained with anti-haCTL II (a–c) and anti-haCTL IV (e–g) antibodies: outer epithelium (a, e), luminal epithelium (b, f), and pouch entrance (c, g). Sections of early-stage brood pouch were immunostained with anti-haCTL II (d) and anti-haCTL IV (h) antibodies. Arrowheads indicate positive signals, and asterisks indicate pigment cells. le, luminal epithelium; oe, outer epithelium. Scale bars = 50 μm. Sections for a–c and e–g were derived from one individual, and those of d and h were derived from another individual