Table 1. Spectroscopy ruler distance measured by DLS and estimated using rigid-rod approximation.

Ruler system description	Ruler distance using DLS/nm	Estimated ruler distance/nm
Dimer separated by 4 bp ds-DNA	3.0 ± 0.2	2.9
Dimer separated by 10 bp ds-DNA	5.0 ± 0.3	4.8
Dimer separated by 20 bp ds-DNA	9.0 ± 0.4	8.0
Dimer separated by 30 bp ds-DNA	12.0 ± 1.0	11.2
Dimer separated by 40 bp ds-DNA	16.0 ± 1.4	14.4
Dimer separated by 60 bp ds-DNA	22.0 ± 2.0	20.8
Dimer separated by 80 bp ds-DNA	26.0 ± 3.0	27.2