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| Ruler system description | λ PR/nm (expt) | λ PR/nm (theory) |
| Dimer separated by 4 bp ds-DNA | 580 | 590 |
| Dimer separated by 10 bp ds-DNA | 550 | 560 |
| Dimer separated by 20 bp ds-DNA | 540 | 545 |
| Dimer separated by 30 bp ds-DNA | 535 | 536 |
| Dimer separated by 40 bp ds-DNA | 532 | 534 |
| Dimer separated by 60 bp ds-DNA | 530 | 530 |
| Dimer separated by 80 bp ds-DNA | 528 | 528 |
