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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Cell Rep. 2017 Sep 26;20(13):3149–3161. doi: 10.1016/j.celrep.2017.08.096

Fig. 3. ATM inflammation precedes visceral fat adipocyte death during DIO.

Fig. 3

Wild-type C57BL/6 mice were fed a low-fat diet (LFD) or 45% high-fat diet (HFD) for 8 or 16 weeks. Panel A: A model for the timing of ATM inflammation and epididymal fat death during DIO. Panel B: Body weight gain. Panel C: mRNA expression of inflammatory cytokines and lipid metabolism genes in purified ATMs. Panel D: Hif1α mRNA levels in epididymal fat (a marker of hypoxia). Panel E: Caspase-3 (CASP3) activity levels in epididymal fat. Panel F: Epididymal fat was stained with antibodies against PLIN2 (red; identifies adipocytes) and MAC2 (green; identifies macrophages), and DAPI (blue; identifies nuclei). Macrophages in crown-like structures (CLS; arrow) were quantified by microscopy and standardized per unit area. Panel G: H&E staining of liver and quantification of liver triglyceride (TG) levels. Results are mean ± SEM; n=5–15, *, p<0.05 Student’s t-test. See also Figs. S3–S4.