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CupA chelates copper from CopY. (A) Inductively coupled plasma mass spectrometry showing the fold increase in the total amount of copper bound to TRV protease-cleaved CupA after chelation of copper from CopY bound to nickel resin. (B) EMSA with 50 ng of pSP_0727, 50 µM CopY, 100 µM Cu2+, and 100 µM wild-type CupA.
