FIG 6 .

LPSs purified from CSCM members induce necroptosis and damage-associated molecular patterns (DAMPs) and IL-33. The CSCM members are A. modestus (Am), A. radioresistens (Ar), D. tsuruhatensis (Dt), and S. maltophilia (Sm). (A) Gene expression of Ripk1 and Ripk3 in LPS-stimulated organoids. (B) Ratio of colonospheres and colonoids during stimulation of organoids derived from WT mice (black bars) and RIPK3^−/− mice (white bars) with LPS purified from CSCM members (1.0 μg/ml). There were eight mice in each group. *, P < 0.05. (C) RT-PCR showing the expression of TNF-α, IL-33, S100A8, S100A9, and S100A14. There were six mice in each group indicated on the x axis. *, P < 0.05 versus nonstimulated control (Ctrl) group.