TABLE 1 .

Mutations in DvH-MO batch culture

Locationa	Locus tag	Gene description/notes	Amino acid change	Nucleotide change
268940		29 bp upstream of DORF26459		A→T
468337		140 bp upstream of DORF27863		C→G
1034706	DVU0942	Ferric uptake regulator (fur)	K→Q	A→C
1118956	DVU1017	ABC transporter, ATP-binding protein/permease protein	A→P	G→C
1213311	DVU1107	Phage tail tape measure protein		G→A
1884231	DVU1819	Protein-export membrane protein SecD	P→A	G→C
2099891	DVU2020	Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase		G→A
2904348	DVU2801	Hypothetical protein	V→G	T→G
2918165		220 bp downstream of DVU2815 outer membrane efflux protein, 573 bp upstream of DVU2812 formate dehydrogenase		C→T
3098151	DVU2990	Molybdopterin biosynthesis MoeA protein	A→G	C→G
P_40,114	DVUA0034	Conserved domain protein; dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis	P→R	G→C
P_92,886	DVUA0070	Conserved domain protein; polysaccharide pyruvyl transferase		A→T

^aReference sequence NCBI GenBank accession no. NC_002937.3 and NC_005863.1. P_40,114 and P_92,886 indicate the positions on the plasmid (bp 40114 and 92886, respectively). The mutation characterized in this study has been highlighted in boldface.