Skip to main content
. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Sci Immunol. 2017 Apr 7;2(10):eaal5237. doi: 10.1126/sciimmunol.aal5237

Figure 5. EBI2-mediated DCIR2+ DC positioning in the outer T cell zone augments induction of Tfh cells.

Figure 5

(A) Time line of DT treatment, cell transfer, immunization, and analysis. OVA-specific OTII T cells were transferred alone (C to F) or together with HEL-specific Hy10 B cells (G and H) into mice containing matched frequencies of control (Ebi2+/−) or Ebi2−/− DCIR2+ DCs (B) Total DCIR2+ DC numbers in mice 3 or 5 days after immunization. (C) Immunofluorescence images of spleen sections showing distribution of CD45.1+ OTII T cells (green), DCIR2+ DCs (red), and B cells (IgD, blue) 12 hours after immunization. (D) Proliferation of OTII cells 3 days after immunization determined by CFSE dilution. (E and F) Up-regulation of ICOS (E) and acquisition of PD-1 and CXCR5 (F) by OTII cells 3 days after immunization. (G) Acquisition of PD-1 and CXCR5 by OTII cells after cotransfer with Hy10 B cells 5 days after immunization. (H) Frequency of total HEL-binding Hy10 cells (left plots) and total number of GL7hiFas+ HEL-binding B cells (graph on right) from the mice in (G). Images in (C) are representative of three mice, and data in (B), (D), (E), and (F) are representative of two independent experiments with at least three mice per group. Data in (G) and (H) are obtained from one experiment with five mice per condition. Scale bars, 100 μm. **P < 0.01; ***P < 0.001; n.s., not significant (P > 0.05) by unpaired Student’s t test.