(A) An eight-tilt EMTdata set (1024 nm by 1280 nm by 180 nm; 141
total TSs, each 1.28 nm thick) of a mitotic chromosome (white box, left panel)
at 29,000× (red box, right panel). Scale bar, 100 nm.To visualize
chromatin ultrastructure and 3D organization as a continuum, see Movie 4. (B) Manual measurements of
chromatin diameter in a single TS. Scale bar, 50 nm. (C) The
mitotic chromosome [red box in (A), 722 nm by 722 nm by 120 nm]
was divided into 36 subvolumes of 120-nm cubes. CVCs are shown in a heat map.
Asterisks indicate cytoplasmic fractions. Scale bar, 100 nm. (D to
F) Surface-thickness estimates of chromatin diameters.There are
two major bin distributions: 5 to 12 nm and 12 to 24 nm (right panel). Scale
bar, 20 nm. (G to I) Continuous erosion analysis to
estimate average chromatin diameter (x-axis intercept).