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. 2017 Apr 6;1(2):166–177. doi: 10.7150/ntno.18531

Figure 2.

Figure 2

Macrophage-targeted theranostic nanoparticle (TNP) for photodynamic therapy of atherosclerosis disease. A) Aqueous solution of TNPs were stable for months without precipitation (pH 7.4) (left), and visualized through MRI (middle) and fluorescence imaging (0.01 mg Fe per mL) (right). B) Cellular uptake and light-induced phototoxicity of the TNPs (i) Flow cytometic analysis of time-dependent accumulation of TNPs as confirmed by cell-associated fluorescence (0.1 mg Fe per mL). (ii) Fluorescence microscopy image shows an uptake and intracellular localization of the TNPs after 3h incubation with RAW 264.7 cells (0.1 mg Fe per mL). (iii) Cell viability of human macrophages after incubation (1 h) with the respective nanoparticles (0.2 mg Fe per mL) and light treatment (42 mW cm-2, 7.5 J), as determined by a MTS assay. The TNP Dark experiment consisted of cells incubated with TNP that did not receive PDT treatment. Control cells were incubated with PBS. All results are relative to the control cells (100 %). (iv) Dose-dependent phototoxicity of TNP in RAW 264.7 cells after incubation (1 h) and light treatment (42 mW cm-2, 7.5 J), as determined by the MTS assay. Reproduced with permission from27.