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. 2017 Jul 7;1(3):326–337. doi: 10.7150/ntno.20233

Figure 2.

Figure 2

The cytotoxicity and affinity of TRAIL in polarized M1 and M2 macrophages by O-glycosylation. (A) Cell morphology of polarized M1 and M2 macrophages after TRAIL (250 ng/ml) treatment for 24 h. Scale bar = 50 μm. Mock indicated the untreated group. (B) The viability of polarized M1 and M2 macrophages after TRAIL (250 ng/ml) treatment for 24 h. (C) The mRNA expressions of DR4 and DR5 for M1 and M2 macrophages. (D) The affinity of M1 and M2 macrophages to TRAIL molecule after incubation with TRAIL (250 ng/ml) for 2 h. The relative protein expression was quantified by the software ImageJ. (E) The gene expression of GLANT14 and FUT6 in M1 and M2 macrophages. (F) The levels of O-GlcNAc proteins for M1 macrophages or M2 macrophages by flow cytometry. Each bar represents the means of three determinations ± SD. *p < 0.05, among the indicated groups.