The in vitro cytotoxicity of gel and the in vivo anticancer activity of drug loaded gels, (A) the cytotoxicity of gelators incubated with NIH/3T3 cells and 4T1 breast cancer cells, the data were expressed as mean ±standard deviation (n=5); (B) the volumes of tumors treated with drug loaded gels; (C) body weights of the mice treated with drug loaded gels; (D) survival rate of tumor-bearing mice; p values at 27 days. (P<0.05 *, P<0.01 **, P<0.001 ***). a Group: saline; b Group: ethanol; c Group: blank gel; d Group: DOX-loaded gel; e Group: DOX·HCl (intratumoral injection); f Group: DOX·HCl (intravenous injection). All the groups were treated by intratumoral injection except for Group f. DOX equivalent dose of 2.5 mg/kg mice. Blank gel was compound 3 in ethanol (10 mg/mL), drug-loaded gel was compound 3 (10 mg/mL) and DOX·HCl (1 mg/mL) in ethanol.