Figure 8.

The CD31, Ki-67 and TUNEL immunohistochemical (IHC) staining of tumor tissues (all tissues: ×400). The brown areas indicated CD31-positive, TUNEL-positive, or Ki-67-positive staining. Representative photomicrographs of 4T1 breast cancer cells harvested at the end of study from mice with various treatment: a Group: saline; b Group: ethanol; c Group: blank gel; d Group: DOX-loaded gel; e Group: DOX·HCl (intratumoral injection); f Group: DOX·HCl (intravenous injection). (Ⅰ: 7th day; Ⅱ: 14th day; Ⅲ: 21th day) All the groups except group f were injected via intratumor. The CD31 positive area was expressed in endothelial cell pack and counted capillary number by reading each section (MVD). The Ki-67 density in each image was calculated by Ki-67-positive area/total area. The apoptotic index was calculated as a ratio of the apoptotic cell number to the total tumor cell number in each field of view. Data were presented as mean ± SD (n = 5). (P<0.05 *, P<0.01 **, P<0.001 ***)