FIG. 8.

PET imaging and biodistribution of Dox-LNP mixed with ⁶⁴Cu-DOTA-cys-DB-LNP. (A) PET imaging of Dox-LNP and ⁶⁴Cu-DOTA-cys-DB-LNP mixture. Dox-LNP was mixed with ⁶⁴Cu-DOTA-cys-DB-LNP and the mixture was injected into two male NOD/SCID mice bearing LnCap tumors. Serial PET imaging was acquired over the 19 hours time course for both mice. Major organs imaged were heart (H), liver (L), and tumor (T). (B) Biodistribution of Dox-LNP and ⁶⁴Cu-DOTA-cys-DB-LNP mixture. After the final PET image was acquired, mice were euthanized, tissues weighed, and counted for radioactivity for biodistribution analysis. The data are expressed for the two individual mice as % ID/g for major organs at the terminal time point of 19 hours. cys-DB, cys-diabody; LNP, lipid nanoparticle; NOD/SCID, non-obese diabetic severe combined immune deficiency; PET, positron emission tomography.