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. 2017 Oct 18;11(10):e0005969. doi: 10.1371/journal.pntd.0005969

Fig 5. The IgG specificities of six commercial antivenoms to venom proteins of six sub-Saharan Africa venoms.

Fig 5

Venoms (10 μg) of B. arietans, E. p. leakeyi, N. nigricollis, N. pallida, N. haje and D. polylepis were separated by reduced SDS-PAGE and visualised by coomassie blue staining (Panel A). Venom proteins in identical gels were transferred to nitrocellulose blots and incubated with 1:5,000 dilutions of naïve Horse IgG (B), the ‘gold standard’ SAIMR polyvalent (C) and SAIMR ECHIS CARINATUS (D) antivenoms, and the ‘test’ Sanofi Pasteur (E), VINS (F), INOSAN (G) and Premium Serums & Vaccines (H) antivenoms. The antivenoms were not standardised to 5 mg/ml as in the ELISA assays.