. 2017 Oct 18;12(10):e0186801. doi: 10.1371/journal.pone.0186801

Table 1. Sequences of primers used for amplification of flanking regions of target genes, pEX18Ap vector backbone and Gm-gfp cassette.

Target gene	Flanking region	Primer sequence 5´-3´
PA1793 (ppiB)	upstream	`GGCAATCGCCAGCGAATTC`
	upstream	`GCGACGAGATCTGTGGGTAATCCGCTTTGTC`
	downstream	`GCGACGGGATCCAGCGATGAGCGTCCTGTTC`
	downstream	`GCGACGAAGCTTCCAGCGGCGCAGGCGCATG`
PA4414 (murD)	upstream	`GGAGGAGAATTCGTACCTGCTGATTCCCAAC`
	upstream	`GGAGGAAGATCTGCTCTCTTCGTCCTCAACG`
	downstream	`GGAGGAAGATCTGATGCTGTCGGTGTTGCG`
	downstream	`GGAGGGAAGCTTCACGCAGACCTTGGCGATC`
PA4416 (murF)	upstream	`GGAGGAGAATTCCTGGAAAAAGTCCTGGAGG`
	upstream	`GGAGGAAGATCTGCGGCACCTCCCAGGCGGC`
	downstream	`GGAGGAAGATCTCTAATGCTCCTGCTGCTGG`
	downstream	`GGAGGGAAGCTTGACTGCCAGAAGTACTTCC`
PA4450 (murA)	upstream	`GGAGGAGAATTCGAGATCCTGCCTTTGCAGG`
	upstream	`GGAGGAAGATCTTGCAATGATCCCCCGTGGG`
	downstream	`GGAGGAAGATCTCGGAGGCTGTCGCGCAAATG`
	downstream	`GGAGGGAAGCTTCTTGATCACGTCGACCTGG`
PA4988 (waaA)	upstream	`GGAGGAGAATTCGAGATGGTCACGGTGTGCG`
	upstream	`GGAGGAAGATCTCATGGGCGCGCAGCTTAGC`
	downstream	`GGAGGAAGATCTGTAGCGCTCGCCCCGTCGG`
	downstream	`GGAGGGAAGCTTCGTGACCATCCAGTGGTTC`
PA5163 (rmlA)	upstream	`GGAGGAGAATTCCGACGAACTCAAGGTCGTG`
	upstream	`GGAGGAAGATCTGTGGTCCCTGCTCGCTCAG`
	downstream	`GGAGGAAGATCTGAAAGCGACCCGCCTGGC`
	downstream	`GGAGGGAAGCTTCCAGAAGTCGGTGGTCTTG`
pEX18Ap vector	Fw	`TATGTACTGTGTTAGCGG`
pEX18Ap vector	Rev	`AAACTCTGGCTCACCG`
Gm-gfp cassette	Fw	`AACTTTGTATAGAGAGCCACTGCG`
Gm-gfp cassette	Rev	`TTAGGTGGCTCAAGTATGGGC`