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. 2017 Oct 6;11(10):e0005965. doi: 10.1371/journal.pntd.0005965

Fig 3. Disruption of the B. bovis hap2 gene and cloning and phenotypic characterization of a hap2 KO strain.

Fig 3

A. Growth curve describing the kinetics of emergence of the transfected parasite line Tf-hap2KO-gfp-bsd upon blasticidin selection. B. Detection of GFP expression by the transfected B. bovis clonal line Tf-hap2KO-gfp-bsd-cln derived from the parasite line Tf-hap2KO-gfp-bsd, left panel is Tf-hap2KO-gfp-bsd-cln and right panel is the non-transfected B. bovis wild type strain T3B. Scale bar: 5 μm. C. Growth curves of the B. bovis Tf-hap2KO-gfp-bsd-cln clonal line and non-transfected B. bovis strain T3B parasite line (wild type) showing similar replication kinetics in vitro without blasticidin selection. The “X” axis represents the percentage of infected erythrocytes in in vitro cultures and the “Y” axis the days after the initiation of the in vitro cultures.