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. 2017 Oct 6;11(10):e0005965. doi: 10.1371/journal.pntd.0005965

Fig 5. Transcription analysis of B. bovis blood stages and induced sexual stages.

Fig 5

A. RT-PCR analysis for the detection of 6-Cys A, 6-Cys B and hap2 transcripts in wild type B. bovis parasites. The RT-PCR analysis was performed using non-induced (NIC) and induced (IC) in vitro cultures. B. 6-Cys A, 6-Cys B RT-PCR products were not detectable in Tf-hap2KO-gfp-bsd-cln induced parasites. Amplifications were performed on samples with (+) or without (-) the addition of reverse transcriptase. RT-PCR amplification of rap1 was used as a positive control. M represents the sizes of molecular markers in base pairs. NIC: non-induced culture, IC: induced culture.