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. 2017 Oct 6;11(10):e0005965. doi: 10.1371/journal.pntd.0005965

Fig 7. Live immunofluorescence analysis for the expression of 6-Cys A and HAP2 in the surface of induced B. bovis extracellular parasites.

Fig 7

A. B. bovis induced cells incubated with anti-6-Cys A and goat anti-rabbit tagged with Alexa Fluor 555 and stained with DAPI; B. B. bovis induced cells incubated anti-HAP2 and goat anti-rabbit tagged with Alexa Fluor 555 and stained with DAPI; C. Negative control preimmune (PI) rabbit serum as primary antibody, and stained with DAPI; D. non-induced B. bovis cells Incubated with Anti 6-Cys A and Anti-HAP2 and goat anti-rabbit tagged with Alexa Fluor 555 and stained with DAP. NIC: non-induced culture, IC: induced culture, Bars, 5 μm.