Table 1. Primers used to amplify the target genes.
Target | Primer forward (5’-3’) | Primer reverse (5’-3’) | Product size (bp) |
---|---|---|---|
hap2 full length | atggatggacccgagaagcgt | caatgggattggatcccgacg | 2271 |
hap2-5’flanking region | ggggctcgagctagaaacccctcaa | ccccctcgagtacatcaacgttggtg | 938 |
hap2-3’flanking region | gggggatcctgtatagagagcacaa | cccggatccatactcttaaactact | 627 |
hap2/ RT- PCR | aaagcgtctatgtaatcaa | acagttttcttctcgtca | 165 |
6-Cys A/ RT- PCR | atggatatccaaaacacattaaatagg | caacatattattcctgtccacacc | 558 |
6-Cys B/ RT- PCR | atgtcgcaattaaacttac | atgtggagtatccgggcc | 500 |