Fig 6. Identification of new IMC proteins in gametocytes.

(A) 3D7 parent and PhIL1-GFP transfectant gametocytes, purified at stage IV, were detergent-solubilized and proteins were immunoprecipitated using GFP-Trap. The input and precipitated eluates (IP) were prepared for SDS-PAGE and Western blot and probed with anti-GFP, anti-GAP50 and anti-GAP45 antibodies. Molecular masses of markers are shown in kDa. (B) List of the PhIL1-interacting proteins. Two independent experiments were performed. Proteins that returned ≥2 significant MS/MS peptides in each experiment are included. A complete list of significant and non-significant proteins identified are presented in S2 Table.