Figure 3.

Injury-induced antimicrobial peptide production is altered in Nod2-deficient mice. (a) Cutaneous β-defensins-1 (mBD-1) and -14 were significantly increased from birth (P0) in Nod2^–/– mouse skin versus WT. (b–e) In adult Nod2^–/– mice, wounding specifically induced both mBD-3 and mBD-14 and IL-22. (f–h) Immunohistochemical analysis showed increased epidermal and dermal mBD-14 at 3 days after wounding in Nod2^–/– mice compared with WT. All data are representative of two or three independent experiments with n = 8 mice/group in a, and n = 5 mice/group in b–h. ^∗∗∗P < 0.001, ^∗∗P < 0.01, ^∗P < 0.05. Mean + standard error of the mean. Scale bar in h = 100 μm. D, day; NS, normal skin; P, postnatal day; WT, wild type.