Figure 3.

The monoclonal antibody V9 epitope in human vimentin corresponds to amino acid residues 411 to 423. Western blot analysis of GST-tagged bacterially expressed human vimentin truncated fragments and an N417T substitution truncated fragment (411–423, 411–423_N417T, 412–422 and 413–421) with the indicated antibodies. Proteins were extracted from E. coli transformed with the pGEX-4T2 constructs after one-hour induction with (+) or without (−) IPTG. Equal amounts of total protein (10 µg) were loaded in each lane.