Figure 5.

Silencing EDIL3 expression interferes with the expression of cell cycle-regulatory proteins and EGFR signaling. HRECs were transfected with siEDIL3 or scramble siRNA and the expression of proteins of interest was detected using western blot analysis. (A) Silencing EDIL3 expression suppressed the protein expression of cyclin D1 and cyclin E1, whereas it had no effect on cyclin B1 expression. (B) Silencing EDIL3 expression appeared to inhibit the phosphorylation of EGFR, Src and ERK in HRECs in vitro. EDIL3, epidermal growth factor-like repeat and discoidin I-like domain-containing protein 3; EGFR, epidermal growth factor receptor; ERK, extracellular signal-regulated kinase; HRECs, human retinal endothelial cells; p-, phosphorylated; si, small interfering.