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Effect of the ERK1/2 inhibitor, U0126, on ALP activity in C2C12 cells. C2C12 cells were cultured for 3 days in the presence or absence of different concentrations and combinations of BMP-2 and FGF-2. Data are expressed as the mean ± standard deviation (n=12). **P<0.01. BMP-2, bone morphogenic protein-2; FGF-2, fibroblast growth factor-2; OD, optical density; ALP, alkaline phosphatase; ERK, extracellular signal-regulated kinase.
