Table I.

Sequence of primers used for reverse transcription-quantitative polymerase chain reaction assay.

Target gene	Primer	Sequence (5′-3′)	Product size (bp)
SOX9	Forward	GGCCGAAGAGGCCACGGAAC	182
	Reverse	TGCCAGCTTGCACGTCGGTT
OPN	Forward	TGAGGCTGCAGTTCTCCTGGC	190
	Reverse	GGGTCAGGCACCAGCCATGT
COLLa1	Forward	CCTGAGTCAGCAGATTGA	106
	Reverse	TCCGCTCTTCCAGTCAG
RUNX2	Forward	GCCAACAAGAGCAGGGAAGGC	146
	Reverse	CTGGCCTGGATTAGGAAGCCAC
OC	Forward	TGAGGACCATCTTTCTGCTCAC	153
	Reverse	CAAGGTAGCGCCGGAGTC
β-actin	Forward	TGCCCAGAAAATGAAAAAGG
	Reverse	GGATGACACAGCGTGAGAGA

RUNX2, runt-related transcription factor 2; SOX9, sex-determining region Y (SRY)-box 9; OC, osteocalcin; OPN, osteopontin; COLLα1, collagen α-1 chain.