Table II.
Primary antibodies used in western blot analysis.
| Antibody | Source | Type | Dilution | Cat. no. | Company |
|---|---|---|---|---|---|
| E-cadherin | Rabbit | Polyclonal | 1:800 | sc7870 | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) |
| Cytokeratin | Rabbit | Polyclonal | 1:500 | sc15367 | Santa Cruz Biotechnology, Inc. |
| N-cadherin | Rabbit | Monoclonal | 1:4,000 | ab76011 | Abcam (Cambridge, MA, USA) |
| Vimentin | Mouse | Monoclonal | 1:500 | sc373717 | Santa Cruz Biotechnology, Inc. |
| α-Smooth muscle actin | Rabbit | Monoclonal | 1:1,000 | ab32575 | Abcam |
| Collagen, type I, α 1 | Rabbit | Polyclonal | 1:1,000 | ab34710 | Abcam |
| Fibroblast growth factor 2 | Rabbit | Polyclonal | 1:500 | ab16828 | Abcam |
| Platelet-derived growth factor BB | Rabbit | Polyclonal | 1:500 | ab23914 | Abcam |
| Rho GTPase activating protein 29 | Rabbit | Polyclonal | 1:2,000 | NBP1-05989 | Novus Biologicals Canada ULC (Oakville, ON, Canada) |
| RhoA | Rabbit | Polyclonal | 1:400 | sc179 | Santa Cruz Biotechnology, Inc. |
| Rho-associated coiled-coil containing protein kinase 1 | Rabbit | Monoclonal | 1:500 | ab45171 | Abcam |
| GAPDH | Rabbit | Monoclonal | 1:2,000 | ab181602 | Abcam |