Figure 1.

5A12 inhibited the proliferation of CD4+ T cells isolated from human peripheral blood without causing toxicity. Cells were stimulated with PHA (A) or CD3/CD28 magnetic beads (B) in the presence of 5A12 for 96 h. The addition of 5A12 to the cultures strongly inhibited proliferation (A and B). CD4+ T cells were stimulated with PHA for 96 h in the presence of 5A12 at 1, 0.5, 0.1, 0.05, 0.01, or 0.001 mg/ml, and the level of apoptosis was evaluated by flow cytometry after staining with FITC-conjugated Annexin V and PI (BioLegend) (C). Data are representative of three independent experiments. Multiple comparisons were performed with one-way ANOVA ***P<0.001.