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. 2017 Aug 4;16(4):4613–4619. doi: 10.3892/mmr.2017.7164

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Copyright: © Qu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Transfection of TRPC1 siRNA reduces spermine-induced increases in [Ca²⁺]_i and NO production in HUVECs. (A) TRPC1 siRNA, but not (B) nonspecific siRNA, reduced the spermine-stimulated increase in [Ca²⁺]_i in the presence of 2 mM [Ca²⁺]_o, compared with (C) the control group. Representative traces are presented in A-C. Bar graphs indicated the effects of siRNA on (D) [Ca²⁺]_i and (E) NO production in HUVECs. ^∆P<0.05 vs. control; ^#P<0.05 vs. nonspecific siRNA. Results are presented as the mean ± standard error of 13–15 cells/test from 7 independent experiments. NO, nitric oxide; siRNA, small interfering RNAs, TRPC1, transient receptor potential canonical 1; HUVECs, human umbilical vein endothelial cells; Fura-2AM, Fura-2-acetoxymethyl ester; DAF-FM, 3 amino, 4 aminomethyl-2,7, difluorescein.