Figure 4.

PDGFRB is a direct target gene of miR-518b. (A) Inverse relationship between the expression of miR-518b and PDGFRB was detected in GBM specimens (magnification, 200x). (B) Western blot analysis of PDGFRB expression in U87 and U251 cells 48 h post-transfection with miR-518b and miR-ctrl. (C) PDGFRB mRNA 3′UTR contains binding sites for miR-518b. (D) U87 and U251 cells were co-transfected with the dual-luciferase reporter plasmid carrying the Wt or Mut 3′UTR sequences of PDGFRB and miR-518b or miR-ctrl mimics. A luciferase reporter system analysis was performed. Data are presented as the mean ± standard deviation (n=5). *P<0.05 vs. miR-ctrl group. miR, microRNA; ctrl, control; PFGFRB, platelet-derived growth factor receptor β; Wt, wild-type; Mut, mutant; 3′UTR, 3′ untranslated region.