Table I.
Sequences of intron-spanning primers for reverse transcription-quantitative polymerase chain reaction.
| Gene | Sequence (5′-3′) | Product size (bp) |
|---|---|---|
| GAPDH | 173 | |
| Forward | TCGGTGTGA ACGGATTTG | |
| Reverse | CTCAGC CTTGACTGTGCC | |
| DGAT1 | 151 | |
| Forward | GCTCCAGCCCATACCCG | |
| Reverse | GCATCACCACGCACCAAT | |
| DGAT2 | 143 | |
| Forward | GAGACTACTTTCCCATCCAG | |
| Reverse | CTTCTTGCTAACTTCGGTG | |
| SREBP-1c | 103 | |
| Forward | GCTCACAAAAGCAAATCACT | |
| Reverse | GCGTTTCTACCACTTCAGG | |
| SREBP-2 | 100 | |
| Forward | CAAACACGGAGAAGTAGACAC | |
| Reverse | CGGGGAAGATAGGAAGG | |
| FAS | 129 | |
| Forward | CTATTGTGGACGGAGGTATC | |
| Reverse | TGCTGTAGCCCAGAAGAG | |
| HMG-CoA R | 137 | |
| Forward | AAGGTGGTGAGAGAAGTATT | |
| Reverse | ATGGCAGTGACGATGTT | |
| PGC-1α | 84 | |
| Forward | ACAAGACTATTGAGCGAACC | |
| Reverse | TGGCTTTATGAGGAGGAG | |
| AdipoR1 | 133 | |
| Forward | GGCTGAAAGACAATGACTACC | |
| Reverse | AGCACAAAACCAAGCAAAT | |
| AdipoR2 | 133 | |
| Forward | TCTTTCTACTGTAACCCAC | |
| Reverse | ACACTCCTGCTCTGACC |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DGAT, diacylglycerol acyltransferase; SREBP, sterol regulatory element binding protein; FAS, fatty acid synthase; HMG-CoA R, 3-hydroxy-3-methyl-glutaryl-CoA reductase; PGC-1α, peroxisome proliferator-activated receptor λ coactivator-1α; AdipoR, adiponectin receptor.