Figure 3.

Investigation of the interaction between PDCD4 and miR-503. (A) The putative miR-503-binding sites in the 3′UTR of PDCD4 and the corresponding mutant binding sites. (B) The relative luciferase activity of luciferase reporter vectors containing Wt or Mut PDCD4-3′UTR in HEK293T cells, which were co-transfected with the miR-503 inhibitor or NC inhibitor. (C) The mRNA levels of PDCD4 in AMC-HN-8 cells transfected with miR-503 inhibitor or NC inhibitor. (D) The protein levels of PDCD4 determined by western blot analysis in AMC-HN-8 cells transfected with miR-503 inhibitor or NC inhibitor. *P<0.05 vs. the respective control. miR, microRNA; NC, negative control; PDCD4, programmed cell death protein 4; UTR, untranslated region; hsa, homo sapiens; Mut, mutant; Wt, wild type.