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. 2017 Aug 11;16(4):5464–5470. doi: 10.3892/mmr.2017.7231

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Copyright: © Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — High expression of miR-20b regulated by human papillomavirus (HPV) E6 in cervical cancer. (A) qPCR analysis of mRNA expression of miR-20b in human cervical tissues. U6 small nuclear RNA was an internal control. Each dot represents 1 sample. Horizontal line is mean, whiskers are SEM. n=19, **P<0.01 vs. vehicle. (B) RT-PCR analysis of the mRNA expression of miR-20b in human cervical cancer cell lines. C33A cells (HPV-) were a control. Data are mean ± SEM (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. control. RT-PCR analysis of mRNA level of (C) HPV18 E6 and (D) miR-20b in HeLa cells after knockdown of HPV18E6 with 2 siRNAs.*P<0.05; **P<0.01.