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. 2017 Aug 11;16(4):5464–5470. doi: 10.3892/mmr.2017.7231

Figure 4.

Figure 4.

HPV and miR-20b regulated the expression of TIMP-2 protein, and miR-20b targeted it. (A) The predicted interaction site of miR-20b and candidate target gene TIMP-2 3′UTR. (B) qPCR analysis of TIMP-2 mRNA level in HeLa cells transfected with miR-106b inhibitor (anti-miR-106b) or miR-106b control inhibitor (anti-NC). Data are mean ± SEM (n=3). (C) Western blot analysis of TIMP-2 protein level in HeLa cells transfected with miR-106b inhibitor (anti-miR-106b) or miR-106b control inhibitor (anti-NC). (D) Luciferase assay of HeLa cells co-transfected with miR-20b mimics and pGL-3-TIMP-2 plasmid (miR-NC and miR-106b with TIMP-2-WT-3′UTR; miR-NC and miR-106b with TIMP-2-Mut-3′UTR) after 24 h. Data are mean ± SEM (n=3). **P<0.01. (E) TIMP-2 protein level after siRNA knockdown of HPV18 E6 protein and (F) quantification. Data are mean ± SEM (n=3). *P<0.05 vs. control, #P<0.05 vs. E6/GAPDH.