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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Cell Rep. 2016 Nov 1;17(6):1546–1559. doi: 10.1016/j.celrep.2016.10.037

Figure 7. GPAT Inhibitor Can Partially Rescue the Differentiation of Seipin−/− Preadipocytes.

Figure 7

(A) Glycerol-3-phosphate dependence of GPAT was determined by incubating 10–20 μg total particulate in the presence of 82.5 μM 16:0 CoA and varying the concentration of G3P from 10 to 800 μM, in the absence or presence of NEM. All experiments were performed in triplicates. Data are presented as mean ± SEM. See also Figure S6.

(B) The recovery of differentiation (day 8) is shown by Oil red O staining of WT, Bscl2 KO, and Bscl2 KO MEF cells treated with 40 μM inhibitor during the first 2 days of differentiation.

(C) Total RNA extracted from wild-type and Seipin−/− preadipocytes cultured in the presence or absence of GPAT inhibitor was used to measure the indicated mRNA levels by qPCR. Values represent mean ± SD of three independent experiments performed in triplicate. Statistical analysis was performed using Student’s t test. *p < 0.05, inhibitor treatment versus DMSO in Seipin KO cells.