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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Methods Mol Biol. 2011;693:163–179. doi: 10.1007/978-1-60761-974-1_10

Fig. 5.

Fig. 5

Transgenic reporter mouse generation. (a–c) Images from TNC-mCherry reporter mice. EGFP was replaced by mCherry to generate pLD53.SC2-mCherry. Three Tenascin C founder lines were generated. All lines showed comparable expression with some variation in reporter gene intensity. (a) Image of TNC-mCherry expression in a E16.5 mouse embryo while still in the yolk sac. Expression is detected in the developing craniofacial structures, developing limbs, and major blood vessels of the yolk sac. (b) Image of TNC-mCherry expression in the mouse foot at E17.5 localized within cartilage condensations. (c) Image of TNC-mCherry expression in the forearm at 2 weeks of age. TNC-mCherry reporter expression is highly expressed in tendon.