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. 2017 Feb 22;174(21):3713–3726. doi: 10.1111/bph.13725

Figure 2.

Figure 2

5FU‐elicited senescence is mediated by p38 and JNK and is associated with a down‐regulation of eNOS and SIRT‐1. (A) Representative pictures of the staining for SA β‐gal after no treatment (CTR) or exposure of EA.hy926 cells to 5FU with or without pre‐incubation with the following signalling inhibitors: SB203580 (inhibitor of p38); SP600125 (inhibitor of JNK); PD98059 (inhibitor of ERK1/2); or LY‐294002 (inhibitor of PI3K). Magnification is 200×, and bars are 50 μm. (B) Percentage of SA β‐gal‐positive EA.hy926 cells in the same conditions as in (A). (C–D) Densitometry analysis and representative western blots for eNOS (C) and SIRT‐1 (D) after the treatments described in (A). eNOS and SIRT‐1 protein levels were expressed as optical density (OD) ratio with actin as internal standard. Data are presented as mean ± SEM of five independent replicates. In all graphs, * indicates statistically significant versus CTR and # statistically significant versus 5FU (ANOVA with post hoc Tukey's multiple comparisons test).