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. Author manuscript; available in PMC: 2018 Oct 18.
Published in final edited form as: J Am Chem Soc. 2017 Oct 5;139(41):14638–14648. doi: 10.1021/jacs.7b07849

Table 2.

Dark state recovery rate constantsa

PixD AppABLUFb
pKac kH2O (s−1) kD2O (s−1) sied kH2O (s−1) kD2O (s−1) sied
Wild-type 9.9 0.038 ± 0.004 0.010 ± 0.0003 3.7 ± 0.1 0.00065 ± 0.00006 0.00013 ± 0.00001 5.0 ± 1
3-FY 8.4 0.22 ± 0.012 0.023 ± 0.0008 9.5 ± 0.1 0.034 ± 0.002 0.0039 ± 0.0001 8.8 ± 0.7
2,3-F2Y 7.8 0.27 ± 0.013 0.047 ± 0.0065 5.8 ± 0.1 0.22 ± 0.01 0.040 ± 0.001 5.5 ± 0.2
3,5-F2Y 7.2 0.58 ± 0.06 0.058 ± 0.0036 9.9 ± 0.2 0.63 ± 0.02 0.11 ± 0.01 5.8 ± 0.3
2,3,5-F3Ye 6.4 ND ND ND 2.6 ± 0.1 1.15 ± 0.03 2.2 ± 0.1
a

Recovery rate constants were obtained from the change in absorption spectra once irradiation at 455 nm had been terminated by fitting the data to a single exponential function. Errors are based on measurements made in triplicate or quadruplicate.

b

AppABLUF. Recovery data are for the BLUF domain of AppA and are taken from Gil et al.21

c

The pKa is that for the amino acid in solution.54

d

sie, solvent isotope effect.

e

ND, not detected. For 2,3,5-F3Y8 PixD no light state was detected.