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. Author manuscript; available in PMC: 2017 Oct 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Sep 19;56(44):13709–13712. doi: 10.1002/anie.201707466

Figure 2. mRNA/PABP nanoplex enhances luciferase expression in a polyamine architecture-dependent manner.

Figure 2

(A) Schematic of synthetic luciferase mRNA with a 120 (A) tail binding to multiple PABP proteins in a tandem manner. One PABP protein binds to 12 continuous adenosine nucleotides. (B) Luciferase mRNA (100 ng) was preassembled with PABP at the indicated molar ratios and transfected with polyamines at a 50:1 N/P ratio. Luciferase expression was quantified 24 hours after transfection into HEK293T cells. The polyamine structures are shown in Supplemental Scheme 1. (C) Fold change in luciferase expression. For each type of polyamine, the increased luciferase expression via delivery of mRNA/PABP was normalized to the expression from delivery of mRNA alone and presented as the fold change. All experiments were performed twice in quadruplicates. Data represent the mean ± SEM (n=4). **, P<0.01; ***, P<0.001; ns, no significance.