Skip to main content
. Author manuscript; available in PMC: 2017 Oct 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Sep 19;56(44):13709–13712. doi: 10.1002/anie.201707466

Figure 3. Side chains of polyamine carriers modulate nano-scale distances that define the functional assembly of mRNA and PABP.

Figure 3

(A) Schematic of stabilized mRNA/PABP complex via polyamines. (B) Representative histograms from the intracellular FRET assay measuring the degree of co-localization between FITC-mRNA and Cy5-PABP. At 24 hours after transfection of Cy5-PABP or FITC-mRNA/Cy5-PABP into HEK293T cells with each polyamine, cells were analyzed by flow cytometry using a 488 nm laser and 695 nm detector with 40 nm bandwidth. The polyamine structures are shown in Supplemental Scheme 1. (C) Mean FRET intensity, calculated by subtracting the fluorescence signal of Cy5-PABP-transfected cells from that of FITC-mRNA/Cy5-PABP-transfected cells. Data represent the mean ± SEM (n=2).