Figure 2.

Comparison of spectral analysis methods for the estimation of blood sO₂ in experimental MSOT images of an animal obtained in vivo and post-mortem. (a–d) eMSOT grid applied on the area of the hindlimb muscle (a) and eMSOT tissue blood sO₂ estimation in the case of 100% O₂ breathing (b), 20% O₂ breathing (c) and post-mortem after CO₂ breathing (d). (e) sO₂ estimation using linear unmixing in the post-mortem case after CO₂ breathing. (f) Normalized spectra, spectral fitting and sO₂ values of linear unmixing (upper row) and eMSOT (lower row) for the three points indicated in (d) and (e). (g) Estimated blood sO₂ of a deep tissue area (yellow box in b) using eMSOT (blue) and linear unmixing (red) under different breathing conditions of CO₂, 20% O₂ and 100% O₂. Image reproduced with permission from [5].