Figure 3.

Species delimitation analysis for genus Mesenchytraeus using a multi-species coalescent paradigm in MrBayes. Shown are results for Coal_set 2 (Suppl. Table 3) comprising 15 genes: five mitochondrial (cytb, nad2/4/5, 12S), five ATP synthase subunits (alpha, gamma, b, c, delta), and five housekeeping (actin, EF-1α, α-tubulin, histone H3, 28S). For this analysis, the mitochondrial genes were considered as a single locus. (a) Depicts the consensus tree with posterior probabilities. (b) Shows a DensiTree2 [38] visualization of the ambiguity present in the consensus tree. This programme uses the full set of trees generated in a Bayesian analysis and overlays them using transparent coloured lies. Branches with greater support among the trees appear darker and heavier while areas of greater topological conflict are more diffuse. Colours are hierarchical with blue lines having highest support, followed by red, and then green.