Fig. 1.

Generation of Dox inducible Mitf reprogrammable system. a A schematic illustration of the Frt-tetO-Mitf knock-in construct directed to the Flpe mCol1a cassette. Also indicated are 3’ Southern blot external probes and the nuclear mCherry transgene marker. b Southern blot verification of tetO-Mitf knock-in to mESCs. Clone #3 was incorrectly targeted, whereas clone #4 was correctly targeted and was used for injection into E3.5blastocyst to obtain ROSA26-M2RtTa^+/+mCol1a-TetO-Mitf ^+/− derived MEFs and somatic cells. c A schematic illustration of the Mitf knock-in reprogrammable MEFs and somatic cells