Fig. 6.

Chemokines promote speed but not directionally persistent T cell migration. GFP⁺ effector T cells were injected i.v. into LPS-treated mice. After two to five days, lungs were explanted and imaged with two-photon microscopy. Identical regions were imaged before and after addition of pertussis toxin (PTX). Tracks with a duration of 15 min were analyzed (n = 205 tracks both before and after treatment). a–c Mean speed (a), time vs. mean squared displacement (MSD) (b), and straightness Z-score (c) before and after treatment with inhibitor. (b) Bars show mean squared displacement. Also shown are standard errors (error bars), linear regression (line) and confidence intervals (shaded areas). Shown are pooled data from experiments with four distinct mice (a, c) or data from a single representative experiment (b). P-values were computed with the Mann–Whitney test