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. 2017 Oct 18;7:13496. doi: 10.1038/s41598-017-13680-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Computer vision based detection of mice iPSC colonies. (a) Example of training patches. Positive samples cropped out as iPSC colonies and negative samples are MEF cells or background. (b) Verification of computer vision based method using the OCT4-GFP reporter. Panels from left to right indicate the bright field original image, a mask of computer detection, an OCT4-GFP fluorescent image, and an overlay between the fluorescence and binary images.