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. 2017 Oct 18;7:13500. doi: 10.1038/s41598-017-13373-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology and expression of neural marker proteins of DPCs. DPCs were transfected with GFP reporter gene using a lenti-viral vector and cultured in the absence or presence of FGF2 (a–h. DPC-S or i–p. DPC-FS, respectively). Cultured DPCs were characterized immunohistochemically using antibodies against GFP (green; b,d,j,l) and/or each indicated neural cell marker (red), respectively: anti-Tuj1- (a,d,i,l), anti-GFAP- (e,m), and anti-MBP- (f,n) and anti-Sox2- (g,o) antibody, respectively. Nuclei were stained with Hoechst 33342 (blue; c–g,k–o). All DPCs showed spindle-shaped fibroblast morphologies (h,p). Scale bar, 100 μm; double bars, 200 μm.