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. 2017 Oct 18;7:13500. doi: 10.1038/s41598-017-13373-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution and quantification of GAP-43–positive area in the spinal cord at 1 week after SCI and DPC transplantation. Fluorescence photomicrographs were GAP-43–immunostained sagittal sections of the spinal cords at 1 mm caudal to the lesion site of the SCI rats at 1 week after transplantation of DPC-S (a) and DPC-FS (b), respectively. Percentages of GAP-43–positive area against total area were evaluated and the values were expressed as means ± SE (c, rostral and d, caudal). The ratio of GAP-43–positive area/total area were significantly larger in the SCI/DPC-FS than those in the SCI/DPC-S group (2.8 ± 0.5 vs 1.0 ± 0.4, for rostral, and 2.9 ± 0.7 vs 1.2 ± 0.5 for caudal; p < 0.05, and p < 0.05, respectively, Student’s t-test, n = 6 for SCI/DPC-S, and n = 9 for SCI/DPC-FS group, respectively). Scale bar 100 μm.