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. 2017 Oct 18;7:13469. doi: 10.1038/s41598-017-14004-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of TGF-β1 on the biosynthesis of PC species as modulated by TGF-βR1 inhibitor. PC synthesis was monitored by ESI-MS/MS in the presence of 10 ng/ml TGF-β1 (black bars) with or without 10 µM TGF-βR1 inhibitor SB431542 (white bars) for 16 hours (n = 5). The percentages of labelled PL species were calculated and then normalized as a ratio compared to the corresponding untreated control. Data are expressed as means ± SDs of the x-fold change of % labelled PL species compared to untreated controls (=1). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; TGF-β1 versus control, or TGF-β1 + SB431542 versus TGF-β1. PC = phosphatidylcholine.