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. 2017 Oct 18;7:13462. doi: 10.1038/s41598-017-13857-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The location of the pemIK-Sa1 operon within single-strain contexts. White arrows indicate sequences that encode hypothetical proteins, and coloured arrows indicate genes encoding: (a) transposase, tn, yellow; CAAX family protease, turquoise; lysophospholipase, brown; primase C family protein, green; a putative DNA-binding protein, dbp, blue; and resolvase, res, yellow. (b) Malate dehydrogenase, orange; putative haemolysin, turquoise; putative immunodominant antigen B, light brown; alkylmercury lyase, blue; beta-lactamase resistance, green; invertase, inv, and resolvase, res, yellow. (c) Invertase, inv; and resolvase, res, yellow; beta-lactamase resistance, green; GNAT family acetyltransferase, turquoise; and 1,4-dihydroxy-2-naphthoate octaprenyltransferase, orange. (d) Protein involved in plasmid mobilisation, mobB, yellow; putative phage protein, orange; CAAX family protease, turquoise; transposase, tn, yellow; teichoic acid ABC transporter permease, blue; putative transcription factor, purple; and cold shock-like protein, cspLA, green. (e) NADH:ubiquinone oxidoreductase, turquoise; replication proteins, rep, olive; yefM /yoeB toxin-antitoxin system, magenta; and MepB protein, mepB, blue. (f) Putative lipoprotein, orange; D-lactate dehydrogenase, turquoise; NAD(P)H:flavin oxidoreductase, blue; putative ring-cleavage extradiol dioxygenase, sky blue; putative acetyltransferase, purple; putative adhesin, grey; MarR family transcription factor, dark green; putative glyoxylase and carboxylesterase, sky blue. (g) Replication protein, repB, olive; primase, turquoise; putative phage protein, blue; and aspartate-semialdehyde dehydrogenase, orange.